My Little Pony and the Gay Agenda
Season 5, episode 9, titled "Slice of Life", as well as "Equestria Girls" short "All's Fair in Love & Friendship Games" heavily imply that the characters 'Lyra' and 'Bonbon' are in a lesbian relationship. In the episode they flirt, have romantic tension, then cuddle and make up at the end of the episode, in a public place no less. The later is entirely centered on their relationship and ends with them embracing as the scene closes in a heart shape. In addition to this, Season 5, episode 17 "Brotherhooves Social" features a male character (Big Macintosh) dressing in drag for much of the episode, visibly disturbing other characters, including children and his own underage sister. In addition, another unnamed male character heavily flirts with him, even showing up at the end of the episode with flowers for Big Mac, but is unable to find him.

Later on, near the end of season 9, the relationship between Lyra and BonBon is confirmed as they propose to one another and are married as a background event. It is likely that since season 9 was the last season, they assumed that any problems that arose from this would not affect them. In addition to this, the character 'Scootaloo' is confirmed to be raised by her lesbian 'aunts' (confirmed in the book "Ponyville Mysteries: Riddle of the Rusty Horseshoe" and by show staff Mike Vogel). Though they seem to be lesbian only in name, as none of their actions in show indicate this. Additionally, Scootaloo's real parents are still alive, but depicted as dismissive and uncaring, further demonizing the straight relationship.